HPLC is Liquid Chromatography used in separating the components of a compound or mixture. HPLC stands for High Performance Liquid Chromatography, and is a technique used to separate different constituents of a compound using high pressure to push solvents through the column. It is the most widely used technique to identify, quantify and separate components of a mixture. HPLC is widely used for the analysis of constituents of a pharmaceutical actives, drug products, pesticides, and countless other substances.